|
| Status |
Public on Mar 01, 2012 |
| Title |
control skin, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
ear skin, control
|
| Organism |
Mus musculus |
| Characteristics |
tissue: ear skin
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ear skin was collected into RNAlater (Ambion, location) and stored at -80 °C prior to analysis. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Genechip 3’ IVT Express Labelling Kit (Affymetrix, Santa Clara, CA, USA) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 7.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT MG-430 PM Array plate. GeneChips were hybridised, washed and stained in the Affymetrix GeneTitan MC instrument.
|
| Scan protocol |
GeneChips were scanned using the scanner in the Affymetrix GeneTitan MC instrument.
|
| Description |
Gene expression data from mouse ear skin.
|
| Data processing |
The data were analyzed by Robust Multichip Analysis using RMA Express [http://rmaexpress.bmbolstad.com]
|
| |
|
| Submission date |
Jan 09, 2012 |
| Last update date |
Mar 01, 2012 |
| Contact name |
Neil A. Mabbott |
| E-mail(s) |
neil.mabbott@roslin.ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
The Roslin Institute & Royal (Dick) School of Veterinary Sciences
|
| Street address |
Easter Bush
|
| City |
Midlothian |
| ZIP/Postal code |
EH25 9RG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE34955 |
Effect of scarification on gene expression in the skin of C57BL/6 mice |
|
