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Sample GSM859518 Query DataSets for GSM859518
Status Public on Mar 01, 2012
Title scraified skin, biological rep3
Sample type RNA
 
Source name ear skin, control
Organism Mus musculus
Characteristics tissue: ear skin
strain: C57BL/6
Extracted molecule total RNA
Extraction protocol Ear skin was collected into RNAlater (Ambion, location) and stored at -80 °C prior to analysis. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared using the Genechip 3’ IVT Express Labelling Kit (Affymetrix, Santa Clara, CA, USA) according to the manufacturer’s instructions.
 
Hybridization protocol Following fragmentation, 7.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT MG-430 PM Array plate. GeneChips were hybridised, washed and stained in the Affymetrix GeneTitan MC instrument.
Scan protocol GeneChips were scanned using the scanner in the Affymetrix GeneTitan MC instrument.
Description Gene expression data from mouse ear skin.
Data processing The data were analyzed by Robust Multichip Analysis using RMA Express [http://rmaexpress.bmbolstad.com]
 
Submission date Jan 09, 2012
Last update date Mar 01, 2012
Contact name Neil A. Mabbott
E-mail(s) neil.mabbott@roslin.ed.ac.uk
Organization name University of Edinburgh
Department The Roslin Institute & Royal (Dick) School of Veterinary Sciences
Street address Easter Bush
City Midlothian
ZIP/Postal code EH25 9RG
Country United Kingdom
 
Platform ID GPL11180
Series (1)
GSE34955 Effect of scarification on gene expression in the skin of C57BL/6 mice

Data table header descriptions
ID_REF
VALUE natural scale RMA expression levels

Data table
ID_REF VALUE
1415670_PM_at 192.351762
1415671_PM_at 507.829504
1415672_PM_at 783.061358
1415673_PM_at 136.569401
1415674_PM_a_at 268.704251
1415675_PM_at 127.577386
1415676_PM_a_at 654.288838
1415677_PM_at 239.619091
1415678_PM_at 568.724519
1415679_PM_at 808.929502
1415680_PM_at 179.799493
1415681_PM_at 421.022622
1415682_PM_at 61.499956
1415683_PM_at 372.532134
1415684_PM_at 84.705059
1415685_PM_at 93.349172
1415686_PM_at 350.230634
1415687_PM_a_at 865.287912
1415688_PM_at 695.61174
1415689_PM_s_at 217.337666

Total number of rows: 45141

Table truncated, full table size 1075 Kbytes.




Supplementary file Size Download File type/resource
GSM859518.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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