|
Status |
Public on Mar 01, 2012 |
Title |
scraified skin, biological rep3 |
Sample type |
RNA |
|
|
Source name |
ear skin, control
|
Organism |
Mus musculus |
Characteristics |
tissue: ear skin strain: C57BL/6
|
Extracted molecule |
total RNA |
Extraction protocol |
Ear skin was collected into RNAlater (Ambion, location) and stored at -80 °C prior to analysis. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared using the Genechip 3’ IVT Express Labelling Kit (Affymetrix, Santa Clara, CA, USA) according to the manufacturer’s instructions.
|
|
|
Hybridization protocol |
Following fragmentation, 7.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HT MG-430 PM Array plate. GeneChips were hybridised, washed and stained in the Affymetrix GeneTitan MC instrument.
|
Scan protocol |
GeneChips were scanned using the scanner in the Affymetrix GeneTitan MC instrument.
|
Description |
Gene expression data from mouse ear skin.
|
Data processing |
The data were analyzed by Robust Multichip Analysis using RMA Express [http://rmaexpress.bmbolstad.com]
|
|
|
Submission date |
Jan 09, 2012 |
Last update date |
Mar 01, 2012 |
Contact name |
Neil A. Mabbott |
E-mail(s) |
neil.mabbott@roslin.ed.ac.uk
|
Organization name |
University of Edinburgh
|
Department |
The Roslin Institute & Royal (Dick) School of Veterinary Sciences
|
Street address |
Easter Bush
|
City |
Midlothian |
ZIP/Postal code |
EH25 9RG |
Country |
United Kingdom |
|
|
Platform ID |
GPL11180 |
Series (1) |
GSE34955 |
Effect of scarification on gene expression in the skin of C57BL/6 mice |
|