|
Status |
Public on Feb 22, 2012 |
Title |
H3K27me3_1'AML_WT |
Sample type |
SRA |
|
|
Source name |
leukemic spleen cells, primary, WT, H3K27me3 ChIP
|
Organism |
Mus musculus |
Characteristics |
background strain: C57BL/6 disease state: MLL-AF9-induced acute myeloid leukemia (AML) cell type: leukemic spleen cells cell origin: WT MLL-AF9 leukemic cell from 1' recipient, sorted for GFP and YFP expression chip antibody: H3K27me3
|
Treatment protocol |
GFP/YFP double-positive cells were isolated by flow sorting from spleens of leukemic mice. Cells were subjected to ChIP using an antibody specific for H3K27me3.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
ChIP DNA libraries were made following the Illumina ChIP-seq library preparation kit.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
H3K27me3_1'AML_WT Chromatin IP against H3K27me3.
|
Data processing |
Sequence reads were aligned to mouse genome assembly mm8 using Bowtie. The ChIP-Seq signal was quantified as total number of reads per million in the region of interest. An empirical background distribution model of reads was constructed to find the significance level of signal in a given region.
|
|
|
Submission date |
Jan 09, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Amit Sinha |
E-mail(s) |
amit.sinha@childrens.harvard.edu
|
Phone |
617-582-7579
|
Organization name |
Dana-Farber Cancer Institute
|
Department |
Pediatric Oncology
|
Lab |
Armstrong Lab
|
Street address |
44 Binney St
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02135 |
Country |
USA |
|
|
Platform ID |
GPL13112 |
Series (2) |
GSE34962 |
Epigenetic profiling of WT and Ezh2-null MLL-AF9 murine leukemic cells |
GSE34963 |
The Polycomb Repressive Complex 2 Is Required For MLL-AF9 Leukemia |
|
Relations |
SRA |
SRX115549 |
BioSample |
SAMN00771667 |
Named Annotation |
GSM859592_WT.H3K27me3.batch2.n_0_l_28_e_70_a_m_1_best_strata.mm8.wig.gz |