Assays to confirm viability: Stratagene mycoplasma PCR test
Growth protocol
Growth medium: ES Cell Medium - DMEM (high glucose) Media supplements: Hyclone FCS, non-essential amino acids, Glutamax, Penicillin/Streptomycin, sodium pyruvate, beta-mercaptoethanol Culture conditions: 37 C, 5% CO2
Extracted molecule
total RNA
Extraction protocol
RNA prep method: RNeasy Cell purification method: Culturing Contaminating cell type: None Method for estimating purity: By eye
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis. Stembase Experiment ID: E201 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=201 SCGP Sample ID: S249 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=201#SAMPLE_216 Short description: 14 days of an 11-point time course study on differentiating embryoid bodies from a murine J1 embryonic stem cell line. The time course includes 0 hr, 6 hr, 12 hr, 18 hr, 24 hr, 36 hr, 48 hr, 4 days, 7 days, 9 days and 14 days. Estimated purity: 100% RNA concentration: 2.0 ug/uL Num cells for RNA prep: Undetermined Sample volume: 15 uL
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
