|
| Status |
Public on May 25, 2014 |
| Title |
no DAPT, day 4 |
| Sample type |
RNA |
| |
|
| Source name |
primary keratinocytes
|
| Organism |
Mus musculus |
| Characteristics |
time: day 4
cell type: primary keratinocyte
treatment: DAPT
genotype: 129, with Pou5f1::GFP transgene
genetic background: 129
|
| Treatment protocol |
Cells were treated with 2.5 uM DAPT for 4 days or 7 days before RNA was harvested
|
| Growth protocol |
Keratinocytes were grown in SFM media from Invitrogen at 30 degrees Celsius and 10% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol LS reagent and extracted according to the Trizol LS protocol from Invitrogen.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
keratinocytes isolated from neonatal mice
|
| Data processing |
The data were normalized using cubic spine with Illumina BeadStudio
|
| |
|
| Submission date |
Jan 13, 2012 |
| Last update date |
May 25, 2014 |
| Contact name |
Justin Ichida |
| E-mail(s) |
cheeda1@gmail.com
|
| Phone |
6172717366
|
| Organization name |
University of Southern California
|
| Street address |
1425 San Pablo St. Bcc 307
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE35090 |
Genome-wide analysis of gene expression changes in mouse keratinocytes induced by treatment with DAPT |
|
