|
| Status |
Public on Dec 07, 2012 |
| Title |
NOD KI Tconv_MLB 022 |
| Sample type |
RNA |
| |
|
| Source name |
NOD KI Tconv
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
strain: NOD/LtJ
genotype: Foxp3tm2Ayr NOD/LtJ
cell type: Tconv
|
| Treatment protocol |
Spleens were isolated from 6 week old Tg(Foxp3-EGFP/cre)1aJbs (WT) and Foxp3tm2Ayr NOD/LtJ mice (KI) (No other treatment given)
|
| Growth protocol |
Cells were lysed directly after sorting
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Spleens were dissociated with frosted glass slides into complete RPMI media supplemented with 10% fetal calf serum. T cells were sorted on CD3+CD4+GFP- and mRNA isolated using TRIZOL
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix 3' IVT Express protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 microgram of cRNA was hybridized for 16 hr at 45C on each array. GeneChips were washed and stained in the Affymetrix GeneTitan system.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan system.
|
| Description |
Foxp3gfp Tconv
|
| Data processing |
The data were analyzed using Partek Genomics Suite 6.5 and summarized by the RMA method
|
| |
|
| Submission date |
Jan 18, 2012 |
| Last update date |
Dec 07, 2012 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE35164 |
Loss of Foxp3-driven epigenetic modification leads to regulatory T cell insufficiency |
|
