•In vitro generated CD8+ central memory(CD8IL-15) T cells were generated from female P14 T cell receptor transgenic mice on the C57Bl/6 genetic background.
•Whole spleen and lymph node cells from female P14 T cell receptor transgenic mice were homogenized and stimulated with cognate peptide (KAVYNFATM) for 2 days. The cells were then grown in IL-15 (central memory/CD8IL-15) for 7 additional days. Central memory (CD8IL-15) CD8+ T cells were sorted by high speed cell sorting for the predominant CD62Lhigh population of cells.
