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Sample GSM86436 Query DataSets for GSM86436
Status Public on Dec 09, 2005
Title mMSC CD34+ 1, chip MG_U74Bv2
Sample type RNA
 
Source name Bone marrow stromal cell clone, Bone marrow
Organism Mus musculus
Characteristics Strain: C57Bl/6
Gender: Female
Defining markers: CD34+, CD117-, Tie-2-,FLK-1-, FLT-1-, CD44-
Growth protocol Growth medium: DMEM
Media supplements: 10% fetal bovine serum
Culture conditions: 37C, 5% CO2
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Cell purification method: Single cell colony isolation
Contaminating cell type: Other bone marrow cells
Method for estimating purity: FACS analysis with CD34+ marker
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Jacques Galipeau (jgalipea@lab.jgh.mcgill.ca; Sir Mortimer B. Davis-Jewish General Hospital; 3755 Cote Ste-Catherine Road) for analysis.
Stembase Experiment ID: E3
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=3
SCGP Sample ID: S4
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=3#SAMPLE_3
Short description: CD34+ cells isolated from adult bone marrow. Subsequently, pure colonies were selected and characterized.
Analysis requested: Compare to control/normal marrow stromal cells previously analyzed for J. Galipeau - Murine genome U74A-C
Estimated purity: 100%
RNA concentration: 0.573 ug/ul
Num cells for RNA prep: 4x10e6
Sample volume: 26.1 ul
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Dec 07, 2005
Last update date Jan 19, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL82
Series (1)
GSE3764 Expression Profile of Homogenous Bone Marrow Stromal Stem Cell Clones

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 149.822 A 0.868639
AFFX-MurIL10_at 778.978 A 0.544587
AFFX-MurIL4_at 413.341 A 0.340661
AFFX-MurFAS_at 582.734 A 0.239063
AFFX-BioB-5_at 6112.81 P 0.0032123
AFFX-BioB-M_at 10901.8 P 9.4506e-05
AFFX-BioB-3_at 8822.06 P 0.000753643
AFFX-BioC-5_at 14541.9 P 0.000195116
AFFX-BioC-3_at 12897.2 P 4.42873e-05
AFFX-BioDn-5_at 19453.7 P 0.000126798
AFFX-BioDn-3_at 83379.9 P 9.4506e-05
AFFX-CreX-5_at 193370 P 4.42873e-05
AFFX-CreX-3_at 256608 P 4.42873e-05
AFFX-BioB-5_st 933.641 A 0.287743
AFFX-BioB-M_st 1695.74 A 0.470241
AFFX-BioB-3_st 568.508 A 0.686277
AFFX-BioC-5_st 262.81 A 0.712257
AFFX-BioC-3_st 171.092 A 0.712257
AFFX-BioDn-5_st 2638.39 A 0.123572
AFFX-BioDn-3_st 3767.31 M 0.0542134

Total number of rows: 12477

Table truncated, full table size 360 Kbytes.




Supplementary file Size Download File type/resource
GSM86436.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM86436.EXP.gz 523 b (ftp)(http) EXP

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