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Sample GSM86443 Query DataSets for GSM86443
Status Public on Dec 09, 2005
Title mMSC CD34+ 3, chip MG_U74Cv2
Sample type RNA
 
Source name Bone marrow stromal cell clone, Bone marrow
Organism Mus musculus
Characteristics Strain: C57Bl/6
Gender: Female
Defining markers: CD34+, CD117-, Tie-2-,FLK-1-, FLT-1-, CD44-
Growth protocol Growth medium: DMEM
Media supplements: 10% fetal bovine serum
Culture conditions: 37C, 5% CO2
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Cell purification method: Single cell colony isolation
Contaminating cell type: Other bone marrow cells
Method for estimating purity: FACS analysis with CD34+ marker
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Jacques Galipeau (jgalipea@lab.jgh.mcgill.ca; Sir Mortimer B. Davis-Jewish General Hospital; 3755 Cote Ste-Catherine Road) for analysis.
Stembase Experiment ID: E3
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=3
SCGP Sample ID: S4
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=3#SAMPLE_3
Short description: CD34+ cells isolated from adult bone marrow. Subsequently, pure colonies were selected and characterized.
Analysis requested: Compare to control/normal marrow stromal cells previously analyzed for J. Galipeau - Murine genome U74A-C
Estimated purity: 100%
RNA concentration: 0.721 ug/ul
Num cells for RNA prep: 4x10e6
Sample volume: 20.8 ul
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Dec 07, 2005
Last update date Jan 19, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL83
Series (1)
GSE3764 Expression Profile of Homogenous Bone Marrow Stromal Stem Cell Clones

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 280.789 A 0.904333
AFFX-MurIL10_at 1749.61 A 0.41138
AFFX-MurIL4_at 397.48 A 0.51489
AFFX-MurFAS_at 192.939 A 0.440646
AFFX-BioB-5_at 6810.51 P 0.00844047
AFFX-BioB-M_at 13779.6 P 0.000972149
AFFX-BioB-3_at 9741.66 P 0.00227496
AFFX-BioC-5_at 20404.4 P 0.000509415
AFFX-BioC-3_at 14431.1 P 0.000126798
AFFX-BioDn-5_at 18163.4 P 0.000296708
AFFX-BioDn-3_at 113296 P 0.000146581
AFFX-CreX-5_at 205622 P 4.42873e-05
AFFX-CreX-3_at 333785 P 4.42873e-05
AFFX-BioB-5_st 742.857 A 0.52976
AFFX-BioB-M_st 547.451 A 0.760937
AFFX-BioB-3_st 464.613 A 0.843268
AFFX-BioC-5_st 527.377 A 0.772364
AFFX-BioC-3_st 614.115 A 0.60308
AFFX-BioDn-5_st 2916.67 A 0.275146
AFFX-BioDn-3_st 3542.78 M 0.0542134

Total number of rows: 11934

Table truncated, full table size 346 Kbytes.




Supplementary file Size Download File type/resource
GSM86443.CEL.gz 2.6 Mb (ftp)(http) CEL
GSM86443.EXP.gz 525 b (ftp)(http) EXP

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