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Status |
Public on Dec 09, 2005 |
Title |
WT3-Expt6, chip MG_U74Bv2 |
Sample type |
RNA |
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Source name |
, Mammary gland
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Organism |
Mus musculus |
Characteristics |
Strain: FVB
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Treatment protocol |
Assays to confirm viability: PCR and immunocytochemistry for sca-1
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Extracted molecule |
total RNA |
Extraction protocol |
RNA prep method: CsCl and Qiagen purification Cell purification method: FACS for Sca-1 +ve Method for estimating purity: PCR for sca-1 transcripts
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Label |
Biotin
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Description |
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. John Hassell (hassell@mcmaster.ca; Life Science Building, Rm 422; 1280 Main St. W.) for analysis. Stembase Experiment ID: E6 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=6 SCGP Sample ID: S65 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=6#SAMPLE_49 Short description: RNA pool from mammary glands tumours of wild-type PEA3 mice induced by the Her2/Neu transgene. Estimated purity: 80% RNA concentration: 2.0 ug/ul Sample volume: 10.0 ul
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Data processing |
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
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Submission date |
Dec 07, 2005 |
Last update date |
Jan 19, 2006 |
Organization |
Ottawa Hospital Research Institute |
Phone |
(613) 737-8899 -73255
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Department |
Cellular and Molecular Medicine
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Lab |
Ottawa Bioinformatics Core Facility
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Street address |
501 Smyth Rd.
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City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1H 8L6 |
Country |
Canada |
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Platform ID |
GPL82 |
Series (1) |
GSE3765 |
Gene Expression Study of Mammary Glands Tumours of PEA3-null and Wild-type PEA3 Mice |
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Data table header descriptions |
ID_REF |
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VALUE |
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06. |
ABS_CALL |
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
DETECTION P-VALUE |
'detection p-value', p-value that indicates the significance level of the detection call |
Data table |
ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
AFFX-MurIL2_at |
165.791 |
A |
0.963431 |
AFFX-MurIL10_at |
343.91 |
A |
0.783476 |
AFFX-MurIL4_at |
76.4704 |
A |
0.957038 |
AFFX-MurFAS_at |
930.573 |
A |
0.368438 |
AFFX-BioB-5_at |
8873.58 |
P |
0.00227496 |
AFFX-BioB-M_at |
18474.9 |
P |
9.4506e-05 |
AFFX-BioB-3_at |
9470.27 |
P |
0.000126798 |
AFFX-BioC-5_at |
24257 |
P |
0.00010954 |
AFFX-BioC-3_at |
26555.3 |
P |
6.02111e-05 |
AFFX-BioDn-5_at |
24966.6 |
P |
6.02111e-05 |
AFFX-BioDn-3_at |
119673 |
P |
4.42873e-05 |
AFFX-CreX-5_at |
247525 |
P |
4.42873e-05 |
AFFX-CreX-3_at |
339288 |
P |
6.59169e-05 |
AFFX-BioB-5_st |
2384.26 |
A |
0.216524 |
AFFX-BioB-M_st |
731.962 |
A |
0.712257 |
AFFX-BioB-3_st |
459.789 |
A |
0.672921 |
AFFX-BioC-5_st |
1246.18 |
A |
0.631562 |
AFFX-BioC-3_st |
89.4504 |
A |
0.631562 |
AFFX-BioDn-5_st |
3242.77 |
A |
0.083592 |
AFFX-BioDn-3_st |
3722.67 |
P |
0.0396608 |
Total number of rows: 12477
Table truncated, full table size 358 Kbytes.
Supplementary file |
Size |
Download |
File type/resource |
GSM86465.CEL.gz |
2.7 Mb |
(ftp)(http) |
CEL |
GSM86465.EXP.gz |
526 b |
(ftp)(http) |
EXP |
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