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Sample GSM86505 Query DataSets for GSM86505
Status Public on Dec 09, 2005
Title OH1, chip MG_U74Bv2
Sample type RNA
 
Source name Mesenchymal cells, Limb bud
Organism Mus musculus
Characteristics Strain: CD-1
Defining markers: Sox9, type II collagen
Treatment protocol Assays to confirm viability: Cells were allowed to differentiate <i>in vitro</i> into chondrocytes
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Cell purification method: Morphology of cultures 3 days post harvest
Contaminating cell type: Fibroblasts
Method for estimating purity: Cells were isolated from the subridge region of the developing limb
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. T. Michael Underhill (tunderhi@uwo.ca; 1400 Western Road) for analysis.
Stembase Experiment ID: E30
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30
SCGP Sample ID: S32
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30#SAMPLE_30
Short description: These samples are multipotential mesenchymal cells.
Estimated purity: 90-95%
Num cells for RNA prep: 6x10e6
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Dec 07, 2005
Last update date Jan 19, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL82
Series (1)
GSE3770 Temporal Analysis of Differentiating Limb Bud Mesenchymal Cell Gene Expression

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 281.874 A 0.868639
AFFX-MurIL10_at 921.074 A 0.699394
AFFX-MurIL4_at 197.95 A 0.645547
AFFX-MurFAS_at 1163.11 A 0.205732
AFFX-BioB-5_at 12566.1 P 0.000445901
AFFX-BioB-M_at 33648.4 P 5.16732e-05
AFFX-BioB-3_at 18177.7 P 4.42873e-05
AFFX-BioC-5_at 34775 P 9.4506e-05
AFFX-BioC-3_at 36957 P 5.16732e-05
AFFX-BioDn-5_at 40096.6 P 5.16732e-05
AFFX-BioDn-3_at 197186 P 5.16732e-05
AFFX-CreX-5_at 397466 P 4.42873e-05
AFFX-CreX-3_at 516223 P 4.42873e-05
AFFX-BioB-5_st 2676.91 A 0.275146
AFFX-BioB-M_st 1277.44 A 0.275146
AFFX-BioB-3_st 2466.92 A 0.340661
AFFX-BioC-5_st 454.548 A 0.876428
AFFX-BioC-3_st 757.196 A 0.52976
AFFX-BioDn-5_st 6422.13 A 0.0629293
AFFX-BioDn-3_st 6246.13 P 0.0138105

Total number of rows: 12477

Table truncated, full table size 357 Kbytes.




Supplementary file Size Download File type/resource
GSM86505.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM86505.EXP.gz 515 b (ftp)(http) EXP

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