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Status
Public on Dec 09, 2005
Title
1D4, chip MG_U74Bv2
Sample type
RNA
Source name
Mesenchymal cells, Limb bud
Organism
Mus musculus
Characteristics
Strain: CD-1 Defining markers: Sox9, type II collagen
Treatment protocol
Assays to confirm viability: Cells were allowed to differentiate <i>in vitro</i> into chondrocytes
Extracted molecule
total RNA
Extraction protocol
RNA prep method: RNeasy Cell purification method: Cells were isolated from the subridge region of the developing limb Contaminating cell type: Fibroblasts Method for estimating purity: Morphology of cultures 3 days post harvest
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/ ). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/ ) by Dr. T. Michael Underhill (tunderhi@uwo.ca; 1400 Western Road) for analysis. Stembase Experiment ID: E30 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30 SCGP Sample ID: S33 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30#SAMPLE_31 Short description: Chondroprogenitors harvested at 1 day. Estimated purity: 90-95% Num cells for RNA prep: 6x10e6
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
Submission date
Dec 07, 2005
Last update date
Jan 19, 2006
Organization
Ottawa Hospital Research Institute
Phone
(613) 737-8899 -73255
Department
Cellular and Molecular Medicine
Lab
Ottawa Bioinformatics Core Facility
Street address
501 Smyth Rd.
City
Ottawa
State/province
ON
ZIP/Postal code
K1H 8L6
Country
Canada
Platform ID
GPL82
Series (1)
GSE3770
Temporal Analysis of Differentiating Limb Bud Mesenchymal Cell Gene Expression
Data table header descriptions
ID_REF
VALUE
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-MurIL2_at
228.552
A
0.794268
AFFX-MurIL10_at
907.973
A
0.5
AFFX-MurIL4_at
161.423
A
0.60308
AFFX-MurFAS_at
943.407
M
0.0584438
AFFX-BioB-5_at
8736.75
P
0.0012475
AFFX-BioB-M_at
15602.4
P
0.000146581
AFFX-BioB-3_at
11135.3
P
0.000169227
AFFX-BioC-5_at
21293.9
P
0.00010954
AFFX-BioC-3_at
19850
P
4.42873e-05
AFFX-BioDn-5_at
20711.8
P
5.16732e-05
AFFX-BioDn-3_at
117169
P
7.00668e-05
AFFX-CreX-5_at
211352
P
4.42873e-05
AFFX-CreX-3_at
289498
P
4.42873e-05
AFFX-BioB-5_st
409.355
A
0.440646
AFFX-BioB-M_st
1147.48
A
0.275146
AFFX-BioB-3_st
866.451
A
0.631562
AFFX-BioC-5_st
296.334
A
0.794268
AFFX-BioC-3_st
185.725
A
0.712257
AFFX-BioDn-5_st
3171.75
A
0.131361
AFFX-BioDn-3_st
5730.27
P
0.036569
Total number of rows: 12477 Table truncated, full table size 360 Kbytes .Supplementary file
Size
Download
File type/resource
GSM86519.CEL.gz
2.7 Mb
(ftp) (http)
CEL
GSM86519.EXP.gz
512 b
(ftp) (http)
EXP