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Sample GSM865223 Query DataSets for GSM865223
Status Public on Dec 23, 2014
Title 13267
Sample type RNA
 
Source name 25W saline + 5W αPlGF
Organism Mus musculus
Characteristics strain: 129S2/SvPasCrl
gender: male
age: 210 days
tissue: liver (healthy)
induction: 25W saline
treatment: 5 weeks αPlGF-treatment
Extracted molecule total RNA
Extraction protocol Qiagen
Label Cy3
Label protocol RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 100 ng of total RNA spiked with 10 viral polyA transcript controls (Agilent) was converted to double stranded cDNA in a reverse transcription reaction. Subsequently the sample was converted to antisense cRNA, amplified and labeled with Cyanine 3-CTP (Cy3) in an in vitro transcription reaction according to the manufacturer’s protocol (Agilent).
 
Hybridization protocol Purified and labeled cRNA (600 ng of Cy3 labeled cRNA) was hybridised on Agilent Sureprint G3 Mouse GE 8x60k Microarrays followed by (manual) washing, according to the manufacturer’s procedures.
Scan protocol To assess the raw probe signal intensities, arrays were scanned using the Agilent DNA MicroArray Scanner with surescan High-Resolution Technology and probe signals were quantified using Agilent’s Feature Extraction software (version 10.7.3.1).
Description 25W saline + 5W αPlGF (treatment) healthy tissue
Data processing The dataset contains the Agilent processed signal values (i.e., feature gProcessedSignal from Agilent Feature Extraction v10.7.3.1) after an additional quantile normalization. Intensities were base 2 log-transformed.
 
Submission date Jan 24, 2012
Last update date Dec 23, 2014
Contact name Rekin's Janky
E-mail(s) Nucleomics.Bioinformatics@vib.be
Organization name VIB
Department Nucleomics Core
Street address Herestraat 49 Box 816
City Leuven
ZIP/Postal code B-3000
Country Belgium
 
Platform ID GPL10787
Series (1)
GSE35289 Placental growth factor as potential target against hepatocellular carcinoma in an orthotopic mouse model.

Data table header descriptions
ID_REF
VALUE The dataset contains the Agilent processed signal values (i.e., feature gProcessedSignal from Agilent Feature Extraction v10.7.3.1) after an additional quantile normalization. Intensities were base 2 log-transformed.

Data table
ID_REF VALUE
A_30_P01017425 5.23347981242131
A_30_P01017426 6.32049097874471
A_30_P01017427 3.19168065681288
A_30_P01017428 6.25582178049465
A_30_P01017429 1.7439403592742
A_30_P01017430 2.70502247953577
A_30_P01017431 3.13555482319552
A_30_P01017432 7.41932253136477
A_30_P01017433 4.66786405378957
A_30_P01017434 5.52826049766423
A_30_P01017435 1.88640243838261
A_30_P01017436 6.51481919222406
A_30_P01017437 5.33002750539378
A_30_P01017438 1.84375666366739
A_30_P01017439 5.17067031131704
A_30_P01017440 1.71635640627011
A_30_P01017441 2.5065555808655
A_30_P01017442 7.72043365298006
A_30_P01017443 8.14005263211259
A_30_P01017444 1.73988618737151

Total number of rows: 51897

Table truncated, full table size 1565 Kbytes.




Supplementary file Size Download File type/resource
GSM865223_US22502572_252800512912_S01_GE1_107_Sep09_2_2.txt.gz 11.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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