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Status
Public on Dec 09, 2005
Title
3D1, chip MG_U74Cv2
Sample type
RNA
Source name
Mesenchymal cells, Limb bud
Organism
Mus musculus
Characteristics
Strain: CD-1 Defining markers: Sox9, type II collagen
Treatment protocol
Assays to confirm viability: Cells were allowed to differentiate <i>in vitro</i> into chondrocytes
Extracted molecule
total RNA
Extraction protocol
RNA prep method: RNeasy Cell purification method: Cells were isolated from the subridge region of the developing limb Contaminating cell type: Fibroblasts Method for estimating purity: Morphology of cultures 3 days post harvest
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/ ). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/ ) by Dr. T. Michael Underhill (tunderhi@uwo.ca; 1400 Western Road) for analysis. Stembase Experiment ID: E30 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30 SCGP Sample ID: S34 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30#SAMPLE_32 Short description: Chondroblasts harvested at 3 days. Estimated purity: 90-95% Num cells for RNA prep: 6x10e6
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
Submission date
Dec 07, 2005
Last update date
Jan 19, 2006
Organization
Ottawa Hospital Research Institute
Phone
(613) 737-8899 -73255
Department
Cellular and Molecular Medicine
Lab
Ottawa Bioinformatics Core Facility
Street address
501 Smyth Rd.
City
Ottawa
State/province
ON
ZIP/Postal code
K1H 8L6
Country
Canada
Platform ID
GPL83
Series (1)
GSE3770
Temporal Analysis of Differentiating Limb Bud Mesenchymal Cell Gene Expression
Data table header descriptions
ID_REF
VALUE
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-MurIL2_at
443.983
A
0.760937
AFFX-MurIL10_at
957.353
A
0.5
AFFX-MurIL4_at
755.109
A
0.131361
AFFX-MurFAS_at
1808.46
M
0.0464816
AFFX-BioB-5_at
9303.82
P
0.00499819
AFFX-BioB-M_at
18938.8
P
0.000195116
AFFX-BioB-3_at
9295.56
P
0.000195116
AFFX-BioC-5_at
23770.8
P
0.000169227
AFFX-BioC-3_at
19862.6
P
5.16732e-05
AFFX-BioDn-5_at
25712.7
P
8.14279e-05
AFFX-BioDn-3_at
149320
P
9.4506e-05
AFFX-CreX-5_at
279195
P
4.42873e-05
AFFX-CreX-3_at
393154
P
4.42873e-05
AFFX-BioB-5_st
1299.65
A
0.216524
AFFX-BioB-M_st
1689.81
A
0.51489
AFFX-BioB-3_st
687.186
A
0.60308
AFFX-BioC-5_st
632.165
A
0.843268
AFFX-BioC-3_st
942.12
A
0.455413
AFFX-BioDn-5_st
4216.85
A
0.102165
AFFX-BioDn-3_st
6376.41
P
0.0182806
Total number of rows: 11934 Table truncated, full table size 346 Kbytes .Supplementary file
Size
Download
File type/resource
GSM86524.CEL.gz
2.7 Mb
(ftp) (http)
CEL
GSM86524.EXP.gz
521 b
(ftp) (http)
EXP