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| Status |
Public on Dec 09, 2005 |
| Title |
PG13 SP2, chip MG_U74Cv2 |
| Sample type |
RNA |
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| Source name |
PG13 cell line, Fibroblast
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| Organism |
Mus musculus |
| Characteristics |
Defining markers: GFP
|
| Treatment protocol |
Assays to confirm viability: 7AAD staining
|
| Growth protocol |
Growth medium: IMDM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA prep method: RNeasy
Cell purification method: Cell sorting
Method for estimating purity: Reanalysis of sorted population by FACS-Vantage
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| Label |
Biotin
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| |
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| Description |
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Mickie Bhatia (mbhatia@robarts.ca; Box 5015; 100 Perth Drive) for analysis.
Stembase Experiment ID: E37
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=37
SCGP Sample ID: S55
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=37#SAMPLE_41
Short description: Total RNA from PG13 transduced with Smad7 recombinant retroviral vector (MIEV-Smad7).
Estimated purity: ~ 98%
RNA concentration: 2.0 ug/ul
Num cells for RNA prep: 4x10e6
Sample volume: 9.0 ul
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| Data processing |
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
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| Submission date |
Dec 07, 2005 |
| Last update date |
Jan 19, 2006 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
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| Department |
Cellular and Molecular Medicine
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| Lab |
Ottawa Bioinformatics Core Facility
|
| Street address |
501 Smyth Rd.
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL83 |
| Series (1) |
| GSE3771 |
Role of Smad7 in Hematopoietic (M-O7e) and Non-Hematopoietic (PG13) Stem Cells |
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| Data table header descriptions |
| ID_REF |
|
| VALUE |
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06. |
| ABS_CALL |
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
| DETECTION P-VALUE |
'detection p-value', p-value that indicates the significance level of the detection call |
| Data table |
| ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
| AFFX-MurIL2_at |
401.568 |
A |
0.814869 |
| AFFX-MurIL10_at |
1206.23 |
A |
0.41138 |
| AFFX-MurIL4_at |
510.745 |
A |
0.794268 |
| AFFX-MurFAS_at |
979.914 |
A |
0.227636 |
| AFFX-BioB-5_at |
19448.1 |
P |
0.000340305 |
| AFFX-BioB-M_at |
41057.5 |
P |
4.42873e-05 |
| AFFX-BioB-3_at |
27241.5 |
P |
4.42873e-05 |
| AFFX-BioC-5_at |
49899.6 |
P |
8.14279e-05 |
| AFFX-BioC-3_at |
44007.6 |
P |
5.16732e-05 |
| AFFX-BioDn-5_at |
51743 |
P |
5.16732e-05 |
| AFFX-BioDn-3_at |
293242 |
P |
7.00668e-05 |
| AFFX-CreX-5_at |
570556 |
P |
4.42873e-05 |
| AFFX-CreX-3_at |
767628 |
P |
4.42873e-05 |
| AFFX-BioB-5_st |
2759.13 |
A |
0.195266 |
| AFFX-BioB-M_st |
597.245 |
A |
0.51489 |
| AFFX-BioB-3_st |
2949.43 |
A |
0.58862 |
| AFFX-BioC-5_st |
658.313 |
A |
0.724854 |
| AFFX-BioC-3_st |
1048.76 |
A |
0.48511 |
| AFFX-BioDn-5_st |
6532.41 |
M |
0.050229 |
| AFFX-BioDn-3_st |
12020.6 |
P |
0.00933744 |
Total number of rows: 11934
Table truncated, full table size 345 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM86548.CEL.gz |
2.6 Mb |
(ftp)(http) |
CEL |
| GSM86548.EXP.gz |
518 b |
(ftp)(http) |
EXP |
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