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Sample GSM865483 Query DataSets for GSM865483
Status Public on Apr 11, 2013
Title H3f3b cKO2 (2) + IRES-GFP
Sample type RNA
 
Source name E12.5 H3f3b Fl/Fl MEFs
Organism Mus musculus
Characteristics strain: C57BL6J
genotype: H3f3b Fl/Fl
age: E12.5
tissue: Mouse Embryonic Fibroblasts
line: cKO2
transduced: GFP vector
Extracted molecule total RNA
Extraction protocol RNA was extracted using Qiagen Rneasy Mini Prep kit. Quality control was performed with Agilent Bioanalyser.
RNA amplification: Illumina® TotalPrep RNA Amplification Kit, manufactured by Ambion, Inc. 
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol-Direct Hybridization
Scan protocol Standard Illumina scanning protocol-Illumina iScan System
Description Replicate C2
Data processing The data were normalized using average normalization using genome studio software
 
Submission date Jan 24, 2012
Last update date Apr 11, 2013
Contact name Paul Knoepfler
E-mail(s) knoepfler@ucdavis.edu
Phone 916-453-2289
Organization name UC Davis
Department Cell Biology and Human Anatomy
Lab Knoepfler Lab
Street address 2425 Stockton Blvd
City Sacramento
State/province CA
ZIP/Postal code 95817
Country USA
 
Platform ID GPL6885
Series (2)
GSE35301 Total Gene expression analysis of H3f3b conditional knockout MEFs
GSE35321 Gene expression changes with loss of H3f3b

Data table header descriptions
ID_REF
VALUE average normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 117 0.73434
ILMN_2707227 152 0.06892
ILMN_2896528 2372.7 0
ILMN_2721178 732.9 0
ILMN_1227723 498 0
ILMN_3033922 2963.1 0
ILMN_3092673 7416.7 0
ILMN_2730714 5547.5 0
ILMN_3162224 1359.2 0
ILMN_2816356 140.5 0.17419
ILMN_2808939 846.2 0
ILMN_2634564 431 0
ILMN_1216623 631.4 0
ILMN_1215157 872 0
ILMN_1228777 107.6 0.9599
ILMN_2737647 131.2 0.33333
ILMN_1216425 116.7 0.74436
ILMN_2734484 457.4 0
ILMN_2652961 106.6 0.96241
ILMN_2952292 212.6 0

Total number of rows: 25697

Table truncated, full table size 629 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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