|
| Status |
Public on Apr 11, 2013 |
| Title |
H3f3b pcKO1 + CRE-IRES-GFP |
| Sample type |
RNA |
| |
|
| Source name |
E12.5 H3f3b Fl/Fl MEFs
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6J
genotype: H3f3b Fl/Fl
age: E12.5
tissue: Mouse Embryonic Fibroblasts
line: pcKO1
transduced: Cre retroviruses
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen Rneasy Mini Prep kit. Quality control was performed with Agilent Bioanalyser.
RNA amplification: Illumina® TotalPrep RNA Amplification Kit, manufactured by Ambion, Inc.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol-Direct Hybridization
|
| Scan protocol |
Standard Illumina scanning protocol-Illumina iScan System
|
| Description |
Replicate F
|
| Data processing |
The data were normalized using average normalization using genome studio software
|
| |
|
| Submission date |
Jan 24, 2012 |
| Last update date |
Apr 11, 2013 |
| Contact name |
Paul Knoepfler |
| E-mail(s) |
knoepfler@ucdavis.edu
|
| Phone |
916-453-2289
|
| Organization name |
UC Davis
|
| Department |
Cell Biology and Human Anatomy
|
| Lab |
Knoepfler Lab
|
| Street address |
2425 Stockton Blvd
|
| City |
Sacramento |
| State/province |
CA |
| ZIP/Postal code |
95817 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE35301 |
Total Gene expression analysis of H3f3b conditional knockout MEFs |
| GSE35321 |
Gene expression changes with loss of H3f3b |
|
