|
Status |
Public on Jul 31, 2014 |
Title |
H3f3b KO 3967 |
Sample type |
RNA |
|
|
Source name |
8 week old H3f3b KO
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL6J genotype: H3f3b -/- age: E12.5 tissue: 1 of 2 mouse testes
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Qiagen Rneasy Mini Prep kit. Quality control was performed with Agilent Bioanalyser. RNA amplification: Illumina® TotalPrep RNA Amplification Kit, manufactured by Ambion, Inc.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol-Direct Hybridization
|
Scan protocol |
Standard Illumina scanning protocol-Illumina iScan System
|
Description |
KO litter 1
|
Data processing |
The data were normalized using average normalization using genome studio software
|
|
|
Submission date |
Jan 24, 2012 |
Last update date |
Jul 31, 2014 |
Contact name |
Paul Knoepfler |
E-mail(s) |
knoepfler@ucdavis.edu
|
Phone |
916-453-2289
|
Organization name |
UC Davis
|
Department |
Cell Biology and Human Anatomy
|
Lab |
Knoepfler Lab
|
Street address |
2425 Stockton Blvd
|
City |
Sacramento |
State/province |
CA |
ZIP/Postal code |
95817 |
Country |
USA |
|
|
Platform ID |
GPL6887 |
Series (2) |
GSE35303 |
Total Gene expression analysis of H3f3b constitutive knockout testis RNA |
GSE35321 |
Gene expression changes with loss of H3f3b |
|