Growth medium: RPMI Media supplements: Penicillin/streptomycin, FBS 409, sodium pyruvate, glutamine, IL-3 Culture conditions: 37C, 95% CO2 for 57 to 110 days
Extracted molecule
total RNA
Extraction protocol
RNA prep method: TRIzol followed by lithium chloride/ethanol precipitation Cell purification method: Growth in IL-3 Contaminating cell type: Possibly bone marrow stromal cells, monocytes, and macrophages Method for estimating purity: Post FACS analysis, morphology
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Kelly McNagny (kelly@brc.ubc.ca; ) for analysis. Stembase Experiment ID: E194 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=194 SCGP Sample ID: S237 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=194#SAMPLE_278 Short description: Mature mast cells cultured for 57 to 110 days. Analysis requested: Expression analysis, murine genome chip MOE430 set comparison of profiles of mature mast cells, mast cell precursors and stem cells, possibly comparisons with other hematopoletic cells Estimated purity: ~80% RNA concentration: 2.0 ug/ul Num cells for RNA prep: 1x10e7 Sample volume: 8.0-10.0 ul
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
