Assays to confirm viability: Undifferentiated antecedants recapitulate mammary gland development.
Growth protocol
Growth medium: High glucose DMEM Media supplements: 5% fetal bovine serum Culture conditions: 37C, 5% CO2; On collagen coated dishes
Extracted molecule
total RNA
Extraction protocol
RNA prep method: TRIzol Cell purification method: Mammospheres cultures induced to differentiate Contaminating cell type: None known Method for estimating purity: Use of molecular markers
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. John Hassell (hassell@mcmaster.ca; Life Science Building, Rm 422; 1280 Main St. W.) for analysis. Stembase Experiment ID: E199 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=199 SCGP Sample ID: S256 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=199#SAMPLE_207 Short description: Differentiated mammosphere RNA. Samples labelled D1, D2 and D4 contain RNA extracted from the cells of mouse mammospheres induced to differentiate over a 6-day period. Analysis requested: Gene Chip Array: MOE 430A, B Estimated purity: 50% myoepithelial, 50% luminal RNA concentration: 2.0 ug/ul Num cells for RNA prep: ~ 8.8x10e6 Sample volume: 5.0 ul
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
