|
| Status |
Public on Jan 28, 2012 |
| Title |
iPS cells from CP stromal |
| Sample type |
RNA |
| |
|
| Source name |
prostate
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPS cells from CP stromal cells
treatment: transfected with NANOG, SOX2, POU5F1, LIN28
|
| Treatment protocol |
Cells were labeled with dye conjugated-antiCD monoclonal antibodies and sorted by MACS. Infected with lentiviral vectors containing NANOG, SOX2, LIN28 and POU5F1/OCT4 constructs.
|
| Growth protocol |
Cells were isolated after overnight tissue digestion with collagenase in serum-free RPMI1640 media. Cells were transfected to produce induced pluripotent stem cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sorted and cultured cell populations were lysed in RLT buffer for RNA isolation.
|
| Label |
biotin
|
| Label protocol |
GeneChip® One-Cycle Target Labeling Kit
|
| |
|
| Hybridization protocol |
Following fragmentation, ten micrograms of adjusted cRNA from each sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
|
| Scan protocol |
Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
|
| Description |
induced pluripotent cells
iPS cells
|
| Data processing |
Raw CEL intensity data were RMA normalized using R/Bioconductor
|
| |
|
| Submission date |
Jan 27, 2012 |
| Last update date |
Jan 28, 2012 |
| Contact name |
Alvin Y Liu |
| E-mail(s) |
aliu@uw.edu
|
| Phone |
206 221-0603
|
| Fax |
206 543-2275
|
| Organization name |
University of Washington
|
| Department |
Urology
|
| Street address |
815 Mercer Street Box 358056
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE35373 |
Reprogramming of prostate cancer-associated stromal cells |
|
