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Sample GSM867832 Query DataSets for GSM867832
Status Public on Sep 04, 2012
Title UTX conditional knockout (cKO) cell line 1
Sample type RNA
Source name embryonic stem cells
Organism Mus musculus
Characteristics cell: ES cells
genotype/variation: UTX conditional knockout
Growth protocol Mouse ES cells were maintained on irradiated embryonic fibroblast feeder cells and cultured in ES medium (DMEM supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 u/ml Penicillin/Streptomycin, 15% ES cell-qualified FBS, and 1000 u/ml LIF (Millipore)). Cells were passaged twice on 0.1% gelatin-coated dishes to remove feeder cells and then cultured in ES medium on gelatin-coated dishes.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
Label biotin
Label protocol standard Affymetrix label protocol
Hybridization protocol standard Affymetrix hyb protocol
Scan protocol standard Affymetrix scan protocol
Description Gene expression data from cKO1 ES cells
Data processing The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
Submission date Jan 30, 2012
Last update date Sep 04, 2012
Contact name Chaochen Wang
Organization name NIDDK
Department LGP
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platform ID GPL1261
Series (1)
GSE35415 UTX regulated genes in mouse embryonic stem cells

Data table header descriptions
VALUE MAS5 signal intensity

Data table
1415670_at 1781.375 P 2.44E-04
1415671_at 3208.3638 P 2.44E-04
1415672_at 5099.6685 P 2.44E-04
1415673_at 3526.5789 P 2.44E-04
1415674_a_at 3386.484 P 2.44E-04
1415675_at 1683.6177 P 2.44E-04
1415676_a_at 11785.776 P 2.44E-04
1415677_at 572.94476 P 7.32E-04
1415678_at 3730.4946 P 2.44E-04
1415679_at 5142.4707 P 2.44E-04
1415680_at 1515.8708 P 2.44E-04
1415681_at 3712.2273 P 2.44E-04
1415682_at 1532.8184 P 2.44E-04
1415683_at 6540.802 P 2.44E-04
1415684_at 663.2904 P 2.44E-04
1415685_at 1456.3027 P 2.44E-04
1415686_at 1530.8478 P 2.44E-04
1415687_a_at 3038.6055 P 2.44E-04
1415688_at 3408.8652 P 2.44E-04
1415689_s_at 517.32654 P 7.32E-04

Total number of rows: 45101

Table truncated, full table size 1423 Kbytes.

Supplementary file Size Download File type/resource
GSM867832.CEL.gz 6.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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