|
| Status |
Public on Sep 04, 2012 |
| Title |
UTX conditional knockout (cKO) cell line 2 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
cell: ES cells
genotype/variation: UTX conditional knockout
|
| Growth protocol |
Mouse ES cells were maintained on irradiated embryonic fibroblast feeder cells and cultured in ES medium (DMEM supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 u/ml Penicillin/Streptomycin, 15% ES cell-qualified FBS, and 1000 u/ml LIF (Millipore)). Cells were passaged twice on 0.1% gelatin-coated dishes to remove feeder cells and then cultured in ES medium on gelatin-coated dishes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix label protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix hyb protocol
|
| Scan protocol |
standard Affymetrix scan protocol
|
| Description |
Gene expression data from cKO2 ES cells
|
| Data processing |
The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
|
| |
|
| Submission date |
Jan 30, 2012 |
| Last update date |
Sep 04, 2012 |
| Contact name |
Chaochen Wang |
| Organization name |
NIDDK
|
| Department |
LGP
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE35415 |
UTX regulated genes in mouse embryonic stem cells |
|
