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Sample GSM867833 Query DataSets for GSM867833
Status Public on Sep 04, 2012
Title UTX conditional knockout (cKO) cell line 2
Sample type RNA
 
Source name embryonic stem cells
Organism Mus musculus
Characteristics cell: ES cells
genotype/variation: UTX conditional knockout
Growth protocol Mouse ES cells were maintained on irradiated embryonic fibroblast feeder cells and cultured in ES medium (DMEM supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 u/ml Penicillin/Streptomycin, 15% ES cell-qualified FBS, and 1000 u/ml LIF (Millipore)). Cells were passaged twice on 0.1% gelatin-coated dishes to remove feeder cells and then cultured in ES medium on gelatin-coated dishes.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
Label biotin
Label protocol standard Affymetrix label protocol
 
Hybridization protocol standard Affymetrix hyb protocol
Scan protocol standard Affymetrix scan protocol
Description Gene expression data from cKO2 ES cells
Data processing The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
 
Submission date Jan 30, 2012
Last update date Sep 04, 2012
Contact name Chaochen Wang
Organization name NIDDK
Department LGP
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL1261
Series (1)
GSE35415 UTX regulated genes in mouse embryonic stem cells

Data table header descriptions
ID_REF
VALUE MAS5 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 1781.7299 P 2.44E-04
1415671_at 3233.2166 P 2.44E-04
1415672_at 5040.4526 P 2.44E-04
1415673_at 4040.3906 P 2.44E-04
1415674_a_at 3977.8245 P 2.44E-04
1415675_at 2118.35 P 2.44E-04
1415676_a_at 12202.566 P 2.44E-04
1415677_at 584.52167 P 2.44E-04
1415678_at 3909.6643 P 2.44E-04
1415679_at 6070.0776 P 2.44E-04
1415680_at 1938.4731 P 2.44E-04
1415681_at 4306.153 P 2.44E-04
1415682_at 1457.718 P 2.44E-04
1415683_at 7603.2217 P 2.44E-04
1415684_at 432.55786 P 2.44E-04
1415685_at 1822.3448 P 2.44E-04
1415686_at 1782.6807 P 2.44E-04
1415687_a_at 2752.2278 P 2.44E-04
1415688_at 3976.452 P 2.44E-04
1415689_s_at 567.7747 P 2.44E-04

Total number of rows: 45101

Table truncated, full table size 1423 Kbytes.




Supplementary file Size Download File type/resource
GSM867833.CEL.gz 6.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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