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Sample GSM867834 Query DataSets for GSM867834
Status Public on Sep 04, 2012
Title UTX knockout (KO) cell line 1
Sample type RNA
 
Source name embryonic stem cells
Organism Mus musculus
Characteristics cell: ES cells
genotype/variation: UTX knockout
Growth protocol Mouse ES cells were maintained on irradiated embryonic fibroblast feeder cells and cultured in ES medium (DMEM supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 u/ml Penicillin/Streptomycin, 15% ES cell-qualified FBS, and 1000 u/ml LIF (Millipore)). Cells were passaged twice on 0.1% gelatin-coated dishes to remove feeder cells and then cultured in ES medium on gelatin-coated dishes.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA followed by Qiagen RNeasy mini kit purification was performed according to the manufacturer's instructions.
Label biotin
Label protocol standard Affymetrix label protocol
 
Hybridization protocol standard Affymetrix hyb protocol
Scan protocol standard Affymetrix scan protocol
Description Gene expression data from KO1 ES cells
Data processing The data were normalized by the methods Microarray Suite version 5.0 (MAS 5.0)
 
Submission date Jan 30, 2012
Last update date Sep 04, 2012
Contact name Chaochen Wang
Organization name NIDDK
Department LGP
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL1261
Series (1)
GSE35415 UTX regulated genes in mouse embryonic stem cells

Data table header descriptions
ID_REF
VALUE MAS5 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 1721.5209 P 2.44E-04
1415671_at 3722.5608 P 2.44E-04
1415672_at 5122.6543 P 7.32E-04
1415673_at 3623.6143 P 7.32E-04
1415674_a_at 3128.6829 P 2.44E-04
1415675_at 1663.5269 P 2.44E-04
1415676_a_at 11467.694 P 2.44E-04
1415677_at 433.538 P 2.44E-04
1415678_at 3384.0454 P 2.44E-04
1415679_at 6168.0806 P 2.44E-04
1415680_at 1360.9601 P 2.44E-04
1415681_at 3420.8433 P 2.44E-04
1415682_at 1341.4193 P 2.44E-04
1415683_at 6201.4004 P 2.44E-04
1415684_at 754.2605 P 2.44E-04
1415685_at 1824.5508 P 2.44E-04
1415686_at 1420.9755 P 2.44E-04
1415687_a_at 2562.0403 P 2.44E-04
1415688_at 3564.9297 P 2.44E-04
1415689_s_at 535.74054 P 7.32E-04

Total number of rows: 45101

Table truncated, full table size 1422 Kbytes.




Supplementary file Size Download File type/resource
GSM867834.CEL.gz 6.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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