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Sample GSM868282 Query DataSets for GSM868282
Status Public on Sep 29, 2012
Title Resting Macrophages-2
Sample type RNA
 
Source name monocyte derived macrophages
Organism Homo sapiens
Characteristics sample type: Random Healthy donors
cell type: macrophages
treatment: none
replicate: Donor 2
Treatment protocol At day 7 cells were treated with IL-4 for 48 hours with 20 ng/ml of IL-4.
Growth protocol Human ciculating monocytes were isolated using a double gradient of Ficall and Percoll, followed by an additional step with the monocyte isolation Kit II of Miltenyi. Cells were cultured in tissue culture plastic for 7 days in X-Viv0 10 medium supplemented with 1% autologous serum.
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicates: Donor 2
Data processing The data were normalised using quantile normalisation in Lumi (R Bioconductor).
 
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL6947
Series (2)
GSE35433 Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE Quantile normalised

Data table
ID_REF VALUE
ILMN_1802380 8.095755893
ILMN_1893287 7.083130766
ILMN_1736104 7.114635804
ILMN_1792389 7.116447986
ILMN_1854015 7.404824286
ILMN_1904757 7.246930529
ILMN_1740305 7.193508193
ILMN_1665168 7.082193994
ILMN_2375156 7.406832014
ILMN_1705423 7.156637528
ILMN_1716072 7.081473195
ILMN_1697642 9.05326836
ILMN_1788184 7.205925506
ILMN_1681845 9.453750453
ILMN_1823296 7.226175571
ILMN_1889845 7.088308618
ILMN_1746923 7.164757393
ILMN_1690979 7.354543453
ILMN_1811114 10.73199373
ILMN_1660729 7.133600601

Total number of rows: 48803

Table truncated, full table size 1186 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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