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Sample GSM868285 Query DataSets for GSM868285
Status Public on Sep 29, 2012
Title IL-4 treated macrophages-2
Sample type RNA
 
Source name monocyte derived macrophages
Organism Homo sapiens
Characteristics sample type: Random Healthy donors
cell type: macrophages
treatment: 48 hours with 20 ng/ml of IL-4
replicate: Donor 2
Treatment protocol At day 7 cells were treated with IL-4 for 48 hours with 20 ng/ml of IL-4.
Growth protocol Human ciculating monocytes were isolated using a double gradient of Ficall and Percoll, followed by an additional step with the monocyte isolation Kit II of Miltenyi. Cells were cultured in tissue culture plastic for 7 days in X-Viv0 10 medium supplemented with 1% autologous serum.
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicates: Donor 2
Data processing The data were normalised using quantile normalisation in Lumi (R Bioconductor).
 
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL6947
Series (2)
GSE35433 Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE Quantile normalised

Data table
ID_REF VALUE
ILMN_1802380 8.302493454
ILMN_1893287 7.227832652
ILMN_1736104 7.107038101
ILMN_1792389 7.222810096
ILMN_1854015 7.517847258
ILMN_1904757 7.14824711
ILMN_1740305 7.078870132
ILMN_1665168 7.083935826
ILMN_2375156 7.475680774
ILMN_1705423 7.143530438
ILMN_1716072 7.126366515
ILMN_1697642 9.153760539
ILMN_1788184 7.230872142
ILMN_1681845 9.879364501
ILMN_1823296 7.15733815
ILMN_1889845 7.184328576
ILMN_1746923 7.241854794
ILMN_1690979 7.332652033
ILMN_1811114 10.02001983
ILMN_1660729 7.141523745

Total number of rows: 48803

Table truncated, full table size 1187 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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