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Sample GSM868290 Query DataSets for GSM868290
Status Public on Sep 29, 2012
Title IL-4 treated macrophages MF-1
Sample type RNA
 
Source name monocyte derived macrophages
Organism Homo sapiens
Characteristics sample type: Random Healthy donors
cell type: macrophages
treatment: 48 hours with 10 ng/ml of IL-4
replicate: Donor 1
Treatment protocol At day 7 cells were treated with IL-4 for 48 hours with 10 ng/ml of IL-4.
Growth protocol Human ciculating monocytes were isolated using a double gradient of Ficall and Percoll, followed by an additional step with the monocyte isolation Kit II of Miltenyi. Cells were cultured in bacteriological plastic for 7 days in X-Viv0 10 medium supplemented with 1% autologous serum.
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicates: Donor 1
Data processing The data were normalised using quantile normalisation in Lumi (R Bioconductor).
 
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL6947
Series (2)
GSE35434 Genome-wide analysis of human macrophages stimulated with IL-4 (10ng/ml) (Illumina)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE Quantile normalised

Data table
ID_REF VALUE
ILMN_1762337 7.809017
ILMN_2055271 8.07929
ILMN_1736007 7.913274
ILMN_2383229 8.041033
ILMN_1806310 7.779695
ILMN_1779670 7.90145
ILMN_2321282 8.267742
ILMN_1671474 7.9679437
ILMN_1772582 8.034352
ILMN_1735698 8.007818
ILMN_1653355 8.136554
ILMN_1717783 7.6631436
ILMN_1705025 7.896075
ILMN_1814316 7.7423315
ILMN_2359168 7.812426
ILMN_1731507 7.7034993
ILMN_1787689 7.859353
ILMN_1745607 12.7634535
ILMN_2136495 7.5390563
ILMN_1668111 7.7312856

Total number of rows: 48803

Table truncated, full table size 1065 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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