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Sample GSM868291 Query DataSets for GSM868291
Status Public on Sep 29, 2012
Title IL-4 treated macrophages MF-2
Sample type RNA
 
Source name monocyte derived macrophages
Organism Homo sapiens
Characteristics sample type: Random Healthy donors
cell type: macrophages
treatment: 48 hours with 10 ng/ml of IL-4
replicate: Donor 2
Treatment protocol At day 7 cells were treated with IL-4 for 48 hours with 10 ng/ml of IL-4.
Growth protocol Human ciculating monocytes were isolated using a double gradient of Ficall and Percoll, followed by an additional step with the monocyte isolation Kit II of Miltenyi. Cells were cultured in bacteriological plastic for 7 days in X-Viv0 10 medium supplemented with 1% autologous serum.
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicates: Donor 2
Data processing The data were normalised using quantile normalisation in Lumi (R Bioconductor).
 
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL6947
Series (2)
GSE35434 Genome-wide analysis of human macrophages stimulated with IL-4 (10ng/ml) (Illumina)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE Quantile normalised

Data table
ID_REF VALUE
ILMN_1762337 8.026594
ILMN_2055271 8.052353
ILMN_1736007 7.90131
ILMN_2383229 7.773047
ILMN_1806310 7.8036823
ILMN_1779670 7.9086084
ILMN_2321282 8.095433
ILMN_1671474 8.032972
ILMN_1772582 7.8260913
ILMN_1735698 8.059321
ILMN_1653355 8.167727
ILMN_1717783 7.77019
ILMN_1705025 7.9421797
ILMN_1814316 7.7828445
ILMN_2359168 7.803388
ILMN_1731507 7.8368635
ILMN_1787689 7.811461
ILMN_1745607 12.814492
ILMN_2136495 7.621132
ILMN_1668111 7.606738

Total number of rows: 48803

Table truncated, full table size 1064 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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