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Sample GSM868293 Query DataSets for GSM868293
Status Public on Sep 29, 2012
Title Resting Macrophages M0 BMMF-1
Sample type RNA
 
Source name Bone Marrow derived macrophages
Organism Mus musculus
Characteristics strain: C57BL/6J
cell type: macrophages
treatment: none
replicate: 1
Treatment protocol Cells were treated for 18 hours with 20 ng/ml of mIL-4.
Growth protocol Mouse bone marrow derived macrophages were prepared by culturing bone marrow extracts for 7 days in RPMI 1640 supplemented with MCSF ( 15 % of L-cell conditioned medium).
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Affymetrix standard protocol
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description Replicate 1
Data processing RMA in Affy R bioconductor
 
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL1261
Series (2)
GSE35435 Genome-wide analysis of mouse macrophages stimulated with IL-4 (bone marrow macrophages) (Affymetrix)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1415670_at 9.190777292
1415671_at 11.84989426
1415672_at 12.12503661
1415673_at 7.779253052
1415674_a_at 10.21315529
1415675_at 10.20734355
1415676_a_at 11.51265519
1415677_at 10.51820339
1415678_at 11.06946844
1415679_at 12.15167674
1415680_at 10.03916863
1415681_at 10.55363342
1415682_at 9.530650888
1415683_at 11.98632347
1415684_at 9.516019087
1415685_at 9.516608517
1415686_at 11.07106067
1415687_a_at 14.8246324
1415688_at 10.9946933
1415689_s_at 8.951265243

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM868293.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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