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Sample GSM868294 Query DataSets for GSM868294
Status Public on Sep 29, 2012
Title Resting Macrophages M0 BMMF-2
Sample type RNA
 
Source name Bone Marrow derived macrophages
Organism Mus musculus
Characteristics strain: C57BL/6J
cell type: macrophages
treatment: none
replicate: 2
Treatment protocol Cells were treated for 18 hours with 20 ng/ml of mIL-4.
Growth protocol Mouse bone marrow derived macrophages were prepared by culturing bone marrow extracts for 7 days in RPMI 1640 supplemented with MCSF ( 15 % of L-cell conditioned medium).
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Affymetrix standard protocol
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description Replicate 2
Data processing RMA in Affy R bioconductor
 
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL1261
Series (2)
GSE35435 Genome-wide analysis of mouse macrophages stimulated with IL-4 (bone marrow macrophages) (Affymetrix)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1415670_at 9.102287309
1415671_at 11.78542627
1415672_at 12.20983647
1415673_at 7.614348947
1415674_a_at 10.19072474
1415675_at 10.22125975
1415676_a_at 11.49174418
1415677_at 10.58731101
1415678_at 11.03339546
1415679_at 12.15125579
1415680_at 9.925472406
1415681_at 10.51950123
1415682_at 9.278299072
1415683_at 12.11313023
1415684_at 9.365081348
1415685_at 9.435820585
1415686_at 11.04157932
1415687_a_at 14.74332306
1415688_at 10.93332219
1415689_s_at 8.987591105

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM868294.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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