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Sample GSM868296 Query DataSets for GSM868296
Status Public on Sep 29, 2012
Title IL-4 treated macrophages M2 BMMF-1
Sample type RNA
 
Source name Bone Marrow derived macrophages
Organism Mus musculus
Characteristics strain: C57BL/6J
cell type: macrophages
treatment: 18 hours with 20 ng/ml of mIL-4
replicate: 1
Treatment protocol Cells were treated for 18 hours with 20 ng/ml of mIL-4.
Growth protocol Mouse bone marrow derived macrophages were prepared by culturing bone marrow extracts for 7 days in RPMI 1640 supplemented with MCSF ( 15 % of L-cell conditioned medium).
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Affymetrix standard protocol
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description Replicate 1
Data processing RMA in Affy R bioconductor
 
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL1261
Series (2)
GSE35435 Genome-wide analysis of mouse macrophages stimulated with IL-4 (bone marrow macrophages) (Affymetrix)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1415670_at 9.215901337
1415671_at 11.64553738
1415672_at 12.07953232
1415673_at 7.489296957
1415674_a_at 10.08998878
1415675_at 10.22087586
1415676_a_at 11.68630533
1415677_at 10.40620958
1415678_at 10.69765318
1415679_at 12.0849317
1415680_at 10.01181563
1415681_at 10.47232207
1415682_at 9.149529816
1415683_at 11.73203383
1415684_at 9.47850301
1415685_at 9.286059889
1415686_at 11.01278451
1415687_a_at 14.8966701
1415688_at 10.4967587
1415689_s_at 8.654528032

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM868296.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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