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Sample GSM868298 Query DataSets for GSM868298
Status Public on Sep 29, 2012
Title IL-4 treated macrophages M2 BMMF-3
Sample type RNA
Source name Bone Marrow derived macrophages
Organism Mus musculus
Characteristics strain: C57BL/6J
cell type: macrophages
treatment: 18 hours with 20 ng/ml of mIL-4
replicate: 3
Treatment protocol Cells were treated for 18 hours with 20 ng/ml of mIL-4.
Growth protocol Mouse bone marrow derived macrophages were prepared by culturing bone marrow extracts for 7 days in RPMI 1640 supplemented with MCSF ( 15 % of L-cell conditioned medium).
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Affymetrix standard protocol
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description Replicate 3
Data processing RMA in Affy R bioconductor
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
Platform ID GPL1261
Series (2)
GSE35435 Genome-wide analysis of mouse macrophages stimulated with IL-4 (bone marrow macrophages) (Affymetrix)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions

Data table
1415670_at 9.280996462
1415671_at 11.84644241
1415672_at 12.25057111
1415673_at 7.968782712
1415674_a_at 10.26864449
1415675_at 10.24038019
1415676_a_at 11.87574639
1415677_at 10.29907486
1415678_at 10.86322328
1415679_at 12.04523048
1415680_at 9.919957382
1415681_at 10.48119906
1415682_at 9.080991639
1415683_at 11.82948998
1415684_at 9.345035859
1415685_at 9.173432712
1415686_at 10.95332776
1415687_a_at 14.8737266
1415688_at 10.72737897
1415689_s_at 8.554783619

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM868298.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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