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Sample GSM868300 Query DataSets for GSM868300
Status Public on Sep 29, 2012
Title Thio permanox M2
Sample type RNA
 
Source name thioglycolate-elicited murine peritoneal macrophages, incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on Permanox plastic
Organism Mus musculus
Characteristics strain: C57BL/6J
replicate: 2
cell type: thioglycolate-elicited murine peritoneal macrophages
treatment: incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on Permanox plastic
Treatment protocol Cells were treated for 24 hours with IL-4 (supernatant of mIL-4 cDNA transfected X63Ag8-653 plasmcytoma cell line).
Growth protocol Mouse thioglycollate and Biogel elicited macrophages were isolated by adhesion and cultured on Pemanox or Tissue culture plastic, in RPMI 1640 .
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Affymetrix standard protocol
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description Replicate 2
Data processing RMA in Affy R bioconductor
 
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL1261
Series (2)
GSE35436 Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1415670_at 9.573813096
1415671_at 12.2702654
1415672_at 12.61531071
1415673_at 9.42888401
1415674_a_at 10.11238886
1415675_at 10.16008655
1415676_a_at 12.71266847
1415677_at 10.31477691
1415678_at 11.19638132
1415679_at 11.91185361
1415680_at 9.95521326
1415681_at 10.87548196
1415682_at 9.366491665
1415683_at 12.04024346
1415684_at 10.07466331
1415685_at 9.527974046
1415686_at 11.33402049
1415687_a_at 14.96209036
1415688_at 10.65244956
1415689_s_at 8.625420157

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM868300.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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