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Sample GSM868302 Query DataSets for GSM868302
Status Public on Sep 29, 2012
Title Thio TCP M2
Sample type RNA
 
Source name thioglycolate-elicited murine peritoneal macrophages, incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic
Organism Mus musculus
Characteristics strain: C57BL/6J
replicate: 1
cell type: thioglycolate-elicited murine peritoneal macrophages
treatment: incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic
Treatment protocol Cells were treated for 24 hours with IL-4 (supernatant of mIL-4 cDNA transfected X63Ag8-653 plasmcytoma cell line).
Growth protocol Mouse thioglycollate and Biogel elicited macrophages were isolated by adhesion and cultured on Pemanox or Tissue culture plastic, in RPMI 1640 .
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Affymetrix standard protocol
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description Replicate 1
Data processing RMA in Affy R bioconductor
 
Submission date Jan 30, 2012
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL1261
Series (2)
GSE35436 Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1415670_at 9.529465557
1415671_at 12.26219119
1415672_at 12.37476118
1415673_at 9.802822804
1415674_a_at 10.33768926
1415675_at 10.15159087
1415676_a_at 12.4630571
1415677_at 10.04664738
1415678_at 11.10559572
1415679_at 11.74866964
1415680_at 9.951444639
1415681_at 10.76414565
1415682_at 9.299217868
1415683_at 12.06474507
1415684_at 9.923982001
1415685_at 9.308917587
1415686_at 11.17518564
1415687_a_at 14.9465799
1415688_at 10.51722059
1415689_s_at 8.448540521

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM868302.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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