|
| Status |
Public on Feb 06, 2012 |
| Title |
MDA-MB-231-pcDNA6 cell culture 2 |
| Sample type |
RNA |
| |
|
| Source name |
cell population of MDA-MB-231 stably transfected with pcDNA6/V5-His plasmid, cell culture 2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: cell line: MDA-MB-231
transfection: pcDNA6/V5-His plasmid
|
| Growth protocol |
Cells were grown in DMEM medium supplemented with 10% fetal bovine serum and 20 µg/ml blasticidin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using the RNeasy Mini Kit according to manufacturer's instructions (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from cell population of MDA-MB-231 stably transfected with pcDNA6/V5-His plasmid, cell culture 2
|
| Data processing |
The data were analyzed with Expression Console (v1.1) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 02, 2012 |
| Last update date |
Feb 06, 2012 |
| Contact name |
Julie A Vendrell |
| E-mail(s) |
julie.vendrell@univ-lyon1.fr
|
| Organization name |
ISPBL - Faculté de Pharmacie de Lyon
|
| Department |
INSERM U1052
|
| Lab |
Centre de Recherche en Cancérologie de Lyon
|
| Street address |
8 avenue Rockefeller
|
| City |
Lyon |
| State/province |
Select a State or Province |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE35511 |
Gene-expression profiling of ZNF217-overexpressing MDA-MB-231 cells |
|
