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Sample GSM870012 Query DataSets for GSM870012
Status Public on Feb 06, 2012
Title MDA-MB-231-pcDNA6 cell culture 2
Sample type RNA
 
Source name cell population of MDA-MB-231 stably transfected with pcDNA6/V5-His plasmid, cell culture 2
Organism Homo sapiens
Characteristics tissue: cell line: MDA-MB-231
transfection: pcDNA6/V5-His plasmid
Growth protocol Cells were grown in DMEM medium supplemented with 10% fetal bovine serum and 20 µg/ml blasticidin
Extracted molecule total RNA
Extraction protocol total RNA was extracted using the RNeasy Mini Kit according to manufacturer's instructions (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description Gene expression data from cell population of MDA-MB-231 stably transfected with pcDNA6/V5-His plasmid, cell culture 2
Data processing The data were analyzed with Expression Console (v1.1) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Feb 02, 2012
Last update date Feb 06, 2012
Contact name Julie A Vendrell
E-mail(s) julie.vendrell@univ-lyon1.fr
Organization name ISPBL - Faculté de Pharmacie de Lyon
Department INSERM U1052
Lab Centre de Recherche en Cancérologie de Lyon
Street address 8 avenue Rockefeller
City Lyon
State/province Select a State or Province
ZIP/Postal code 69008
Country France
 
Platform ID GPL570
Series (1)
GSE35511 Gene-expression profiling of ZNF217-overexpressing MDA-MB-231 cells

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 1002.888
1053_at 1491.284
117_at 54.00422
121_at 1314.571
1255_g_at 7.58179
1294_at 319.2812
1316_at 81.83609
1320_at 116.0408
1405_i_at 4.466692
1431_at 43.85629
1438_at 3.931736
1487_at 1131.211
1494_f_at 131.8928
1552256_a_at 776.1558
1552257_a_at 1809.332
1552258_at 138.7987
1552261_at 25.06739
1552263_at 507.3091
1552264_a_at 949.2493
1552266_at 12.1909

Total number of rows: 54675

Table truncated, full table size 1052 Kbytes.




Supplementary file Size Download File type/resource
GSM870012.CEL.gz 5.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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