|
| Status |
Public on Nov 12, 2013 |
| Title |
child-22 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood sample
|
| Organism |
Homo sapiens |
| Characteristics |
age (yr): 10.6
gender: M
disease status: asthmatic
|
| Growth protocol |
Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner
|
| Description |
Gene expression data from peripheral blood
|
| Data processing |
The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
|
| |
|
| Submission date |
Feb 06, 2012 |
| Last update date |
Nov 13, 2013 |
| Contact name |
Rebecca Catherine Fry |
| E-mail(s) |
rfry@unc.edu
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Environmental Sciences and Engineering
|
| Street address |
1213 MHRC
|
| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE35571 |
Gene expression data from 131 human subjects in Detroit, Michigan |
|
