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Sample GSM870919 Query DataSets for GSM870919
Status Public on Nov 12, 2013
Title child-27
Sample type RNA
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 11.8
gender: M
disease status: asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
1007_s_at 6.910931424
1053_at 6.835593475
117_at 10.13755071
121_at 7.169553807
1255_g_at 2.753081099
1294_at 9.028744683
1316_at 4.714680092
1320_at 3.439326279
1405_i_at 10.20763109
1431_at 3.288715054
1438_at 4.789379344
1487_at 8.273972478
1494_f_at 4.814818579
1552256_a_at 7.167757345
1552257_a_at 7.691456566
1552258_at 4.514516957
1552261_at 3.48554027
1552263_at 8.625567772
1552264_a_at 9.483906459
1552266_at 2.623295498

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.

Supplementary file Size Download File type/resource
GSM870919.CEL.gz 4.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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