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Sample GSM870935 Query DataSets for GSM870935
Status Public on Nov 12, 2013
Title child-51
Sample type RNA
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 10.7
gender: M
disease status: non-asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
1007_s_at 7.155014351
1053_at 6.716741197
117_at 9.507254944
121_at 7.136518857
1255_g_at 2.861976665
1294_at 9.196473996
1316_at 4.779507954
1320_at 3.486199797
1405_i_at 11.15013799
1431_at 3.431094883
1438_at 4.618878854
1487_at 8.351882123
1494_f_at 5.091769246
1552256_a_at 7.415342177
1552257_a_at 8.019530987
1552258_at 4.945664569
1552261_at 3.832148162
1552263_at 8.551566154
1552264_a_at 9.170494039
1552266_at 2.507312056

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.

Supplementary file Size Download File type/resource
GSM870935.CEL.gz 4.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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