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Sample GSM870943 Query DataSets for GSM870943
Status Public on Nov 12, 2013
Title child-64
Sample type RNA
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 9.7
gender: M
disease status: non-asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
1007_s_at 6.56796581
1053_at 6.764316905
117_at 9.619134053
121_at 6.846288335
1255_g_at 2.500159281
1294_at 8.762241939
1316_at 4.504898063
1320_at 3.720419885
1405_i_at 12.42818368
1431_at 3.396990129
1438_at 4.537124892
1487_at 8.138268857
1494_f_at 5.154856291
1552256_a_at 6.548776758
1552257_a_at 7.448526819
1552258_at 4.784093431
1552261_at 3.786660903
1552263_at 8.698885006
1552264_a_at 9.557107031
1552266_at 2.419002307

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.

Supplementary file Size Download File type/resource
GSM870943.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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