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Sample GSM870954 Query DataSets for GSM870954
Status Public on Nov 12, 2013
Title child-77
Sample type RNA
 
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 9.9
gender: M
disease status: asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
 
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
E-mail(s) rfry@unc.edu
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
 
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
ID_REF
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
ID_REF VALUE
1007_s_at 7.027658504
1053_at 6.7651258
117_at 9.526825675
121_at 7.334867502
1255_g_at 2.665878672
1294_at 9.156260331
1316_at 4.845277071
1320_at 3.455459843
1405_i_at 12.23257948
1431_at 3.706299865
1438_at 4.712209711
1487_at 8.371921398
1494_f_at 5.133111086
1552256_a_at 7.367692954
1552257_a_at 7.78446865
1552258_at 5.04618149
1552261_at 3.569983804
1552263_at 8.73300922
1552264_a_at 9.509901895
1552266_at 2.574020814

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM870954.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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