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Sample GSM870958 Query DataSets for GSM870958
Status Public on Nov 12, 2013
Title child-82
Sample type RNA
 
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 10.9
gender: M
disease status: asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
 
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
E-mail(s) rfry@unc.edu
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
 
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
ID_REF
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
ID_REF VALUE
1007_s_at 7.22279832
1053_at 6.786890354
117_at 9.241061207
121_at 7.305030498
1255_g_at 2.682226741
1294_at 8.980303289
1316_at 4.850983051
1320_at 3.638407543
1405_i_at 11.04566649
1431_at 3.358685458
1438_at 4.460297688
1487_at 8.039414042
1494_f_at 5.33579998
1552256_a_at 6.799191029
1552257_a_at 7.312333865
1552258_at 4.599414335
1552261_at 3.412077211
1552263_at 8.554301131
1552264_a_at 9.398476766
1552266_at 2.789873124

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM870958.CEL.gz 4.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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