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Sample GSM870963 Query DataSets for GSM870963
Status Public on Nov 12, 2013
Title child-87
Sample type RNA
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 11.2
gender: M
disease status: asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
1007_s_at 7.076208027
1053_at 6.803770673
117_at 9.628435034
121_at 7.102722295
1255_g_at 2.691630264
1294_at 8.986778568
1316_at 4.438339693
1320_at 3.524154326
1405_i_at 10.89981401
1431_at 3.67130816
1438_at 4.875978557
1487_at 8.166045721
1494_f_at 5.106010525
1552256_a_at 6.788057518
1552257_a_at 7.42843019
1552258_at 4.408848098
1552261_at 3.22806062
1552263_at 8.535328379
1552264_a_at 9.287459201
1552266_at 2.459541145

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.

Supplementary file Size Download File type/resource
GSM870963.CEL.gz 4.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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