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Sample GSM870964 Query DataSets for GSM870964
Status Public on Nov 12, 2013
Title child-88
Sample type RNA
 
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 11.7
gender: M
disease status: non-asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
 
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
E-mail(s) rfry@unc.edu
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
 
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
ID_REF
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
ID_REF VALUE
1007_s_at 7.063708301
1053_at 6.434457518
117_at 9.849198327
121_at 6.879556921
1255_g_at 2.546090028
1294_at 9.00305393
1316_at 4.585679204
1320_at 3.43577473
1405_i_at 10.58199693
1431_at 3.447869827
1438_at 4.337826386
1487_at 7.901496375
1494_f_at 5.118188256
1552256_a_at 6.692389912
1552257_a_at 7.645297793
1552258_at 4.870596243
1552261_at 3.188504774
1552263_at 8.346392674
1552264_a_at 9.151891543
1552266_at 2.824624618

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM870964.CEL.gz 4.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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