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Sample GSM870973 Query DataSets for GSM870973
Status Public on Nov 12, 2013
Title child-99
Sample type RNA
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 13.5
gender: M
disease status: asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
1007_s_at 6.931386716
1053_at 6.556049181
117_at 10.22154116
121_at 6.897319474
1255_g_at 2.654555695
1294_at 8.439581825
1316_at 4.628793532
1320_at 3.551985376
1405_i_at 10.47676029
1431_at 3.41576138
1438_at 4.506460273
1487_at 7.811751376
1494_f_at 5.274083681
1552256_a_at 6.574580008
1552257_a_at 7.605931759
1552258_at 4.439110281
1552261_at 3.083299885
1552263_at 8.807602425
1552264_a_at 9.359557247
1552266_at 2.222562366

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.

Supplementary file Size Download File type/resource
GSM870973.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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