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Sample GSM870981 Query DataSets for GSM870981
Status Public on Nov 12, 2013
Title child-114
Sample type RNA
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 13.4
gender: M
disease status: non-asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
1007_s_at 6.95727288
1053_at 6.822629331
117_at 9.622509492
121_at 7.059839906
1255_g_at 2.680783717
1294_at 8.667809346
1316_at 4.811278951
1320_at 3.474115713
1405_i_at 10.94920143
1431_at 3.447503861
1438_at 4.320181466
1487_at 8.283252221
1494_f_at 5.302186799
1552256_a_at 6.434247042
1552257_a_at 7.598379211
1552258_at 4.489311819
1552261_at 3.295933637
1552263_at 9.040684624
1552264_a_at 9.822150365
1552266_at 2.586934061

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.

Supplementary file Size Download File type/resource
GSM870981.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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