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Sample GSM870989 Query DataSets for GSM870989
Status Public on Nov 12, 2013
Title child-125
Sample type RNA
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 11.2
gender: F
disease status: asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
1007_s_at 6.976412487
1053_at 6.859393524
117_at 9.303240861
121_at 6.837271817
1255_g_at 2.800004453
1294_at 8.731660013
1316_at 4.611569267
1320_at 3.671402869
1405_i_at 11.2697884
1431_at 3.381253087
1438_at 4.569447715
1487_at 7.90813572
1494_f_at 5.242246736
1552256_a_at 6.610786848
1552257_a_at 7.446088076
1552258_at 4.560721169
1552261_at 3.298299044
1552263_at 8.734649114
1552264_a_at 9.399676975
1552266_at 2.654651118

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.

Supplementary file Size Download File type/resource
GSM870989.CEL.gz 4.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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