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Sample GSM870997 Query DataSets for GSM870997
Status Public on Nov 12, 2013
Title child-140
Sample type RNA
 
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 12.4
gender: F
disease status: non-asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
 
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
E-mail(s) rfry@unc.edu
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
 
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
ID_REF
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
ID_REF VALUE
1007_s_at 6.989949634
1053_at 6.901581393
117_at 10.27842873
121_at 7.262810129
1255_g_at 2.861337819
1294_at 8.741918345
1316_at 4.738925164
1320_at 3.543706689
1405_i_at 10.52551382
1431_at 3.528436213
1438_at 4.341576297
1487_at 7.989034212
1494_f_at 4.972712898
1552256_a_at 6.37024473
1552257_a_at 7.47026631
1552258_at 4.578411046
1552261_at 3.464622146
1552263_at 8.859361946
1552264_a_at 9.627729001
1552266_at 2.497103353

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM870997.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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