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Sample GSM871023 Query DataSets for GSM871023
Status Public on Nov 12, 2013
Title child-187
Sample type RNA
 
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 13.5
gender: M
disease status: asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
 
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
E-mail(s) rfry@unc.edu
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
 
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
ID_REF
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
ID_REF VALUE
1007_s_at 7.304583008
1053_at 6.711459291
117_at 10.27097055
121_at 7.709467731
1255_g_at 3.016185904
1294_at 8.798619685
1316_at 4.748233552
1320_at 4.038748937
1405_i_at 9.359866899
1431_at 3.551864363
1438_at 4.704473955
1487_at 7.859368652
1494_f_at 5.39405452
1552256_a_at 6.97673523
1552257_a_at 7.781775712
1552258_at 4.462178176
1552261_at 3.713861307
1552263_at 8.532326535
1552264_a_at 9.250591402
1552266_at 2.62398432

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM871023.CEL.gz 4.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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