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Sample GSM871031 Query DataSets for GSM871031
Status Public on Nov 12, 2013
Title child-196
Sample type RNA
Source name peripheral blood sample
Organism Homo sapiens
Characteristics age (yr): 10.0
gender: F
disease status: asthmatic
Growth protocol Peripheral blood samples were collected in PAXgene blood RNA tubes (Qiagen, Valencia, CA, USA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen PAXgene Blood RNA Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization protocol following fragmentation. cRNA was hybridized on to the Affymetrix U133 2.0 Plus Human Array
Scan protocol GeneChips were scanned using the Affymetrix Scanner
Description Gene expression data from peripheral blood
Data processing The normalization was a quantile normalization per the canonical Robust Multi-Chip method. Each sample was used as a stand alone expression value, but those values were normalized to provide a common distribution using the quantile normalization (at the probe level) and summarized using the median polish algorithm (to produce a single log2 transformed value per probe set).
Submission date Feb 06, 2012
Last update date Nov 13, 2013
Contact name Rebecca Catherine Fry
Organization name UNC-Chapel Hill
Department Environmental Sciences and Engineering
Street address 1213 MHRC
City Chapel Hill
ZIP/Postal code 27599
Country USA
Platform ID GPL570
Series (1)
GSE35571 Gene expression data from 131 human subjects in Detroit, Michigan

Data table header descriptions
VALUE Robust Multi-Chip Analysis signal intensity (log2)

Data table
1007_s_at 6.862453513
1053_at 6.973050802
117_at 10.10655375
121_at 7.247458045
1255_g_at 2.731643777
1294_at 8.715136563
1316_at 4.840479902
1320_at 3.606583467
1405_i_at 10.18738518
1431_at 3.685579493
1438_at 4.498948064
1487_at 8.039290104
1494_f_at 5.146106982
1552256_a_at 6.430495046
1552257_a_at 7.458221553
1552258_at 4.717103901
1552261_at 3.694501618
1552263_at 8.924489123
1552264_a_at 9.332601412
1552266_at 2.918999472

Total number of rows: 54662

Table truncated, full table size 1211 Kbytes.

Supplementary file Size Download File type/resource
GSM871031.CEL.gz 4.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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