|
Status |
Public on Jul 08, 2013 |
Title |
TNBS colitis day 14_mouse#2 |
Sample type |
RNA |
|
|
Source name |
distal colon
|
Organism |
Mus musculus |
Characteristics |
strain: BALB/c disease status: TNBS colitis time: day 14 animal: mouse#2
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared using Illumnia TotalPrep RNA Amplification Biotinylated cRNA were prepared using Illumnia TotalPrep RNA Amplification Biotinylated cRNA were prepared using Illumnia TotalPrep RNA Amplification
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate _2
|
Data processing |
Image Analysis and extraction of raw and background subsracted expression data were preformed with Genome Studio software. GeneSpring GX 11.0 was used for quantile normalization and converting expression levels to a log2 scale
|
|
|
Submission date |
Feb 07, 2012 |
Last update date |
Jul 08, 2013 |
Contact name |
Rob Mariman |
E-mail(s) |
rob.mariman@tno.nl
|
Organization name |
TNO
|
Street address |
Vestwal 5
|
City |
Leiden |
ZIP/Postal code |
2312NP |
Country |
Netherlands |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE35609 |
Analysis of temporal gene expression responses during TNBS-induced colitis development |
|